A laboratory technician is preparing to quantify a newly synthesized dye

CHEMISTRY · Multi-part · AP · Chemistry · Properties of Substances and Mixtures · Properties of Substances and Mixtures

Problem

A laboratory technician is preparing to quantify a newly synthesized dye. For the chosen analytical wavelength, the dye’s molar absorptivity is

1.87 \times 10^4\,\text{L mol}^{-1}\,\text{cm}^{-1}

. A quartz cuvette with an internal path length of

0.850\,\text{cm}

is employed. A solution that is known to be

3.20 \times 10^{-5}\,\text{M}

in the dye is placed in the cuvette, and its absorbance is recorded. Answer the following. (1) Part 1: (a) Calculate the expected absorbance of the solution. Show your work. (2) Part 2: (b) The spectrophotometer’s detector is linear only for absorbance values between

0.050

and

1.000

. Based on your calculation in part (a), determine whether the measured absorbance will fall within the instrument’s linear range and justify your answer.

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