An experiment on a zinc-containing enzyme requires a buffer that (1) maintains pH 7.0…
CHEMISTRY · Single correct · A Level · Chemistry · Acids and bases · Acids and bases
Problem
An experiment on a zinc-containing enzyme requires a buffer that (1) maintains pH 7.0 at 37 °C, (2) does not bind Zn²⁺, and (3) can be used at 0.10 M concentration. Data for four common buffers are listed.
Buffer pKa at 25 °C ΔpKa/°C Metal-ion binding
(a) Tris
(b) Phosphate
(c) PIPES
(d) HEPES