A researcher needs a buffer to maintain pH 7.4 during a cell-culture experiment
CHEMISTRY · Single correct · A Level · Chemistry · Acids and bases · Acids and bases
Problem
A researcher needs a buffer to maintain pH 7.4 during a cell-culture experiment. Which conjugate acid–base pair is most appropriate for this purpose?
(a) imidazolium/imidazole (pKa=6.99)
(b) HEPES acid/HEPES base (pKa=7.55)
(c) carbonic acid/bicarbonate (pKa=6.37)
(d) dihydrogen phosphate/monohydrogen phosphate (pKa=7.21)