A donor and acceptor dye pair with Forster radius is attached to two points on a…

PHYSICS · Subjective · Fermi University Physics · Biophysics & Medical Physics · Macromolecules, membranes and cell architecture · Macromolecules, membranes and cell architecture

Forces, Machines and Signals, Ch Seeing molecules: diffraction, cryo-electron microscopy and fluorescence

Problem

A donor and acceptor dye pair with Forster radius R0=5.4 nmR_0 = 5.4\,\mathrm{nm} is attached to two points on a folded RNA. The measured FRET efficiency is E=0.65E = 0.65. Using E=1/[1+(r/R0)6]E = 1/[1+(r/R_0)^6], find the donor-acceptor distance rr, and state whether it lies within the usable range 0.5R0<r<1.5R00.5R_0 < r < 1.5R_0 for this dye pair.

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