A single-particle cryo-EM reconstruction is checked by splitting the data into two…
PHYSICS · Multi-part · Fermi University Physics · Biophysics & Medical Physics · Macromolecules, membranes and cell architecture · Macromolecules, membranes and cell architecture
Forces, Machines and Signals, Ch Seeing molecules: diffraction, cryo-electron microscopy and fluorescence
Problem
A single-particle cryo-EM reconstruction is checked by splitting the data into two independent half-maps and computing the Fourier shell correlation (FSC) between them as a function of spatial frequency q. Data as pairs (q in nm−1, FSC): (0.10,0.98), (0.15,0.85), (0.20,0.60), (0.25,0.35), (0.30,0.18), (0.32,0.143), (0.35,0.08).
((a)) Using the standard 0.143 threshold, read off the spatial frequency at which the map's resolution is set.
((b)) Convert this spatial frequency into a real-space resolution in nanometres.