A microscope has a objective of numerical aperture (object side) and is used…
PHYSICS · Multi-part · Fermi University Physics · Optics & Photonics · Fourier optics and image formation · Fourier optics and image formation
What an Instrument Can See, Ch The transfer function of an imaging system
Problem
A microscope has a 100× objective of numerical aperture NA=1.20 (object side) and is used incoherently at λ=500nm.
((a)) Compute the incoherent cutoff frequency at the object.
((b)) State the largest camera pixel pitch that avoids aliasing, given the Nyquist spacing at the object and the 100× magnification to the camera.