A microscope objective has on the object side and a field number of measured at the…

PHYSICS · Multi-part · Fermi University Physics · Optics & Photonics · Fourier optics and image formation · Fourier optics and image formation

What an Instrument Can See, Ch The transfer function of an imaging system

Problem

40\times

microscope objective has

\mathrm{NA} = 0.75

on the object side and a field number of

25\ \mathrm{mm}

measured at the image plane; it is used incoherently at

\lambda = 550\ \mathrm{nm}

. ((a)) Find the diameter, and hence the area, of the imaged field at the object. ((b)) Compute the incoherent cutoff frequency at the object. ((c)) Compute the space-bandwidth product

\mathrm{SBP} = A\pi\nu_c^2

of the irradiance image.

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