A confocal microscope objective has numerical aperture and is used with light of…
PHYSICS · Multi-part · Fermi University Physics · Optics & Photonics · Fourier optics and image formation · Fourier optics and image formation
What an Instrument Can See, Ch The transfer function of an imaging system
Problem
A confocal microscope objective has numerical aperture NA=0.95 and is used with light of vacuum wavelength λ=488nm (an argon-ion laser line), referred to the object plane.
((a)) State the coherent transfer function's cutoff frequency, in cycles per millimetre.
((b)) State the incoherent (optical transfer function) cutoff frequency, in cycles per millimetre.
((c)) Give the Rayleigh resolution figure for this objective and wavelength, in nanometres, naming the criterion.