A water-immersion fluorescence objective has and is used at

PHYSICS · Multi-part · Fermi University Physics · Optics & Photonics · Fourier optics and image formation · Fourier optics and image formation

What an Instrument Can See, Ch The transfer function of an imaging system

Problem

A water-immersion fluorescence objective has

\mathrm{NA} = 1.20

and is used at

\lambda = 520\ \mathrm{nm}

, imaging a circular field of view of diameter

0.15\ \mathrm{mm}

at the object. ((a)) Give the Abbe, Rayleigh and Sparrow resolution figures for this objective and wavelength, in nanometres, each with its criterion named. ((b)) Compute the space-bandwidth product

\mathrm{SBP} = A\pi\nu_c^2

of the irradiance (incoherent) image over the stated field, where

A

is the field area and

\nu_c

is the incoherent cutoff.

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